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Permeabilization of the Naegleria fowleri plasmatic membrane to the vital dye SYTOX® green caused by incubation with the IC90 of STS for 24 h (C,D). Negative control (A,B). Images (40×) are representative of the cell population observed in the performed experiments. Images were obtained using an EVOS FL Cell Imaging System AMF4300, Life Technologies, USA. Obtained results were similar for both N. fowleri type strains.
