Table 1.
Inhibitory concentrations (IC50) of natural ICZs (1-4) evaluated against the trophozoite stage of Naegleria fowleri type strains ATCC® 30808™ and ATCC® 30215™ and toxicity values (CC50) against murine macrophage J774A.1. NA indicates that no activity was observed. (Mean concentration ± standard deviation (SD)).
| Compound | N. fowleri ATCC® 30808™ IC50 (µM) | N. fowleri ATCC® 30215™ IC50 (µM) | Murine Macrophage J774.A1 CC50 (µM) | SI * (CC50/IC50) | Log P ** |
|---|---|---|---|---|---|
| STS (1) | 0.08 *** ± 0.01 | 0.08 **** ± 0.02 | 8.74 ± 0.72 | 109.3 | 3.86 |
| 7OSTS (2) | 1.28 ± 0.35 | 1.07 ± 0.23 | 5.20 ± 1.75 | 4.1 | 3.74 |
| 4′D4′OSTS (3) | 10.79 ± 2.92 | NA | >20 | >1.9 | 3.38 |
| SCZ B (4) | 11.51 ± 0.31 | NA | >20 | >1.7 | 3.43 |
| Amphotericin B | 0.12 ± 0.03 | 0.16 ± 0.02 | ≥200 | ≥1666.7 | - |
| Miltefosine | 38.74 ± 4.23 | 81.57 ± 7.23 | 127.88 ± 8.85 | 3.3 | - |
* Refers to Naegleria fowleri ATCC® 30808™; ** Calculated with ChemDraw (version 19.1.0.5), PerkinElmer Informatics, Inc.; *** IC90 = 0.58 ± 0.16; **** IC90 = 0.97 ± 0.14.