Figure 2.

In situ immunofluorescence localization of tomato brown rugose fruit virus (ToBRFV) and pepino mosaic virus (PepMV) in symptomatic tomatoes. (a1) ToBRFV coat protein (CP) detection in infected tomato seeds using antibodies conjugated to Alexa 488, visualized with green channel; (a2) ToBRFV-infected tomato seeds visualized using bright field; (a3) ToBRFV-infected tomato seeds visualized by merging channels; (b1) PepMV CP detection in tomato seeds using antibodies conjugated to Alexa 594, visualized with red channel; (b2) PepMV-infected tomato seeds visualized using bright field; (b3) PepMV-infected tomato seeds visualized by merging channels; (c1) ToBRFV-CP detection in co-infected tomato exocarp, facing the mesocarp, using the green channel; (c2) PepMV-CP detection in the co-infected tomato exocarp, facing the mesocarp, using the red channel; (c3) co-infected tomato exocarp, facing the mesocarp, visualized using bright field; (c4) ToBRFV and PepMV detection in co-infected tomato exocarp, facing the mesocarp, using merged channels; (d1) ToBRFV-CP detection in co-infected tomato exocarp surface using the green channel; (d2) PepMV-CP detection in co-infected tomato exocarp surface using the red channel; (d3) Co-infected tomato exocarp surface visualized using bright field; (d4) Exocarp surface from a co-infected tomato visualized using merged channels; (e,f) Exocarp dissected from a control healthy tomato subjected to immunofluorescence. (e1) Imaging of exocarp facing the mesocarp using the green channel; (e2) Imaging of exocarp facing the mesocarp using the red channel; (e3) Imaging of exocarp facing the mesocarp using bright field; (e4) Imaging of exocarp facing the mesocarp using merged channels; (f1) Imaging of exocarp surface using the green channel; (f2) Imaging of exocarp surface using the red channel; (f3) Imaging of exocarp surface using bright field; (f4) Imaging of exocarp surface using merged channels; (g) Exocarp dissection from a co-infected tomato fruit.