. Author manuscript; available in PMC: 2020 Jun 10.

Published in final edited form as: Curr Opin Immunol. 2018 Dec 19;56:100–106. doi: 10.1016/j.coi.2018.11.003

Table 1: Murine ILC progenitors in vitro analysis.

This table represents insight into the single cell in vitro culture conditions used in mice to discover lineage restriction and subsequent results.

Cell	Identification (Lin^-)	Progenitor Location	Transcription factors	Stromal Cell use	Length of culture (days)	Culture Cytokines	NK cell	ILC1	ILC2	ILC3	Refs
αLP	CXCR6⁺α4β7⁺CD127⁺	BM	Tox, Nfil3	OP9-DL1	21	KL, IL-7	NK1.1⁺Eomes⁺Tbet⁺	NK1.1⁺Eomes^-Tbet⁺	CD127⁺Gata3⁺ Rorγt^-	CD127⁺Gata3^-Rorγt⁺	8
EILP	Thy1.2^-α4β7⁺CD127^-	BM	Tox, Nfil3, Tcf7	OP9	10	KL, IL-7, IL-2	NK1.1⁺Gata3^-NKp46⁺DX5⁺	NK1.1⁺Gata3^-NKp46⁺DX5^-	NK1.1^-Gata3⁺	NK1.1^-Gata3^-NKp46^-Rorγt⁺	9
CHILP	CD117⁺α4β7⁺CD127⁺CD25^-Id2⁺	BM	Tox, Tcf7, Id2	OP9-DL1	14	KL, IL-7	NK1.1⁺Eomes⁺^**	NKp46⁺Tbet⁺Rorγt^-	NKp46^-Gata3⁺Rorγt^-	NKp46^+/-Rorγt⁺	10
ILCP	CD127⁺α4β7⁺Plzf⁺	BM	Tox, Tcf7, Id2, Plzf	OP9, OP9-DL1	10–12	KL, IL-7	NK1.1⁺CD49a^-CD49b⁺^**	NK1.1⁺Bcl11b^-	NK1.1^-Bcl11b⁺	NK1.1^-Bc11b^-Rorγt⁺	15, 16

^**

indicates population was not tested for in single cell cultures