Table 2: Human ILC progenitors in vitro analysis.
Cell | Identification (Lin-) | Transcription factors | Stromal cell use | Length of culture (days) | Cytokines | NK cell | ILC1 | ILC2 | ILC3 | Refs |
---|---|---|---|---|---|---|---|---|---|---|
ETP1 | CD34+CD117-CD94-CD10+ | Tox | OP9-DL1 | 28 | IL-3, IL-7, KL, FL, IL-15 | CD94+IFN-γ+ | CD94-CD161+IFN-γ+ | CD94-CD161+IL-13+ | CD94-CD161+IL-22+ | 22, 29 |
ETP2 | CD34+CD117+IL-1R1-CD94- | Tox, Id2, Nfil3 | OP9-DL1 | 28 | IL-3, IL-7,KL, FL, IL-15 | CD94+IFN-γ+ | CD94-CD161+IFN-γ+ | CD94-CD161+IL-13+ | CD94-CD161+IL-22+ | 22, 29 |
CILP | CD34+CD117+IL-1R1+CD94- | Tox, Id2, Nfil3, AHR, Rorγt | OP9-DL1 | 28 | IL-3, IL-7, KL, FL, IL-15 | CD94+IFN-γ+ | CD94-CD161+IFN-γ+ | CD94-CD161+IL-13+ | CD94-CD161+IL-22+ | 22, 29 |
ILCP | CD34-CD117+IL-1R1+CD94- | Tox, Tcf7, Id2, Plzf | OP9, OP9-DL4 | 14–18 | IL-2, IL-7, IL-1β, IL-23 | CD7+CD94+Eomes+* | CD7+CD94-Eomes-IFN-γ+* | CD7+CD94-IL-13+ | CD94-CD7+IL-22+and/or CD7+IL-17A+ | 27 |
rILCP | CD34-CD117+IL-1R1+CD56+ | Tcf7, Plzf | OP9-DL1 | 28 | IL-2, IL-7, KL, FL | CD94+ | ** | CD94-CD294+ | CD94-NKp44+ | 28 |
Indicates populations were not distinguished from one another in single cell cultures
Indicates population was not detected or tested for during this study