. Author manuscript; available in PMC: 2020 Jun 10.

Published in final edited form as: Curr Opin Immunol. 2018 Dec 19;56:100–106. doi: 10.1016/j.coi.2018.11.003

Table 2: Human ILC progenitors in vitro analysis.

This table represents insight into the single cell in vitro culture conditions used in humans to determine lineage restriction and subsequent results.

Cell	Identification (Lin^-)	Transcription factors	Stromal cell use	Length of culture (days)	Cytokines	NK cell	ILC1	ILC2	ILC3	Refs
ETP1	CD34⁺CD117^-CD94^-CD10⁺	Tox	OP9-DL1	28	IL-3, IL-7, KL, FL, IL-15	CD94⁺IFN-γ⁺	CD94^-CD161⁺IFN-γ⁺	CD94^-CD161⁺IL-13⁺	CD94^-CD161⁺IL-22⁺	22, 29
ETP2	CD34⁺CD117⁺IL-1R1^-CD94^-	Tox, Id2, Nfil3	OP9-DL1	28	IL-3, IL-7,KL, FL, IL-15	CD94⁺IFN-γ⁺	CD94^-CD161⁺IFN-γ⁺	CD94^-CD161⁺IL-13⁺	CD94^-CD161⁺IL-22⁺	22, 29
CILP	CD34⁺CD117⁺IL-1R1⁺CD94^-	Tox, Id2, Nfil3, AHR, Rorγt	OP9-DL1	28	IL-3, IL-7, KL, FL, IL-15	CD94⁺IFN-γ⁺	CD94^-CD161⁺IFN-γ⁺	CD94^-CD161⁺IL-13⁺	CD94^-CD161⁺IL-22⁺	22, 29
ILCP	CD34^-CD117⁺IL-1R1⁺CD94^-	Tox, Tcf7, Id2, Plzf	OP9, OP9-DL4	14–18	IL-2, IL-7, IL-1β, IL-23	CD7⁺CD94⁺Eomes⁺^*	CD7⁺CD94^-Eomes^-IFN-γ⁺^*	CD7⁺CD94^-IL-13⁺	CD94^-CD7⁺IL-22⁺and/or CD7⁺IL-17A⁺	27
rILCP	CD34^-CD117⁺IL-1R1⁺CD56⁺	Tcf7, Plzf	OP9-DL1	28	IL-2, IL-7, KL, FL	CD94⁺	^**	CD94^-CD294⁺	CD94-NKp44⁺	28

Indicates populations were not distinguished from one another in single cell cultures

^**

Indicates population was not detected or tested for during this study