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. 2020 Jun 9;16:186. doi: 10.1186/s12917-020-02415-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Ultrastructural changes in BAEC exposed to P. multocida B:2. a Severe nuclear invagination (long arrow), with dilatation, fragmentation, and vesiculation of RER (short arrows). Few mitochondria had cristae disarrangement and partial cristolysis (arrowhead), with low number of P. multocida B:2 (asterisk) in the cytoplasm. (bar = 2 μm, TEM). b Disintegration of plasma membrane and nuclear envelope (long arrows), with prominent swollen mitochondria and cristae disarrangement (short arrow) and RER vesiculation (arrow head) (bar = 2 μm, TEM). c Presence of numerous P. multocida B:2 (asterisks) inside and outside of a lysed BAEC (long arrow). Mitochondria cristolysis (short arrow) and ribosomal degranulation from RER membranes with accumulation of ribosomal fragments in the cytosol were also noted (arrow heads) (bar = 2 μm, TEM). d. Presence of lysed (long arrow) and intact (short arrows) P. multocida B:2 with shedding of outer membrane (arrowheads). (bar = 500 nm, TEM)