Fig. 3.

The effects of silibinin on NSCLC cell behaviors and the expressions of RHBDD1 and EMT markers. A549 cells were treated with silibinin (10, 20 and 40 μM) or vehicle (DMSO) for 24 h. a Cell proliferation was analyzed using the CCK-8 assay. b Transwell migration and invasion assays were performed in A549 cells. c The protein expressions of RHBDD1, E-cadherin, N-cadherin and vimentin were determined using western blotting. Data are expressed as means ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, compared with DMSO control