Fig. 5.

Silibinin affects NSCLC cell proliferation, migration and invasion by regulating RHBDD1. RHBDD1 overexpression plasmid, si-RHBDD1 and the relevant negative control (NC) were transfected into A549 cells, followed by treatment with 40 μM silibinin. a Cell proliferation was analyzed using the CCK-8 assay. b Transwell migration and invasion assays were performed in A549 cells. c The protein expressions of RHBDD1, E-cadherin, N-cadherin and vimentin were determined using western blotting. Data are expressed as means ± standard deviation (SD). **p < 0.01, ***p < 0.001, compared with silibinin + NC