Fig. 6.

P. acnes-induced MIF production via the NF-κB pathway. aP. acnes supernatant could activate both the NF-κB and ERΚ1/2 pathways in a time-dependent manner. b, c The ERK1/2 pathway is inhibited by FR180204, and the NF-κB pathway is inhibited by BAY-7082. d, e Western blot analysis of MIF expression infected with P. acnes supernatant for 48 h, pretreated with or without FR-180204 (20 μM). f, g Western blot analysis of MIF expression infected with P. acnes supernatant for 24 h, pretreated with or without BAY-7082 in a concentration-dependent manner. *p < 0.05, **p < 0.01, ***p < 0.001. p values were analyzed by two-sided Student’s t test. The data are expressed as means ± SD