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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: J Am Chem Soc. 2019 Aug 6;141(32):12824–12831. doi: 10.1021/jacs.9b05912

Table 2.

Properties of BODIPY VoltageFluor (VF) dyes

Name R R1 isomer λmax absa λmax ema ϕfla % ΔF/Fb,c Cell brightnessc,d
6 EtpH Et H para 528 541 0.14 0 0.43 ± 0.02f
7 EtpOMe Et OMe para 527 541 0.07 0 0.76 ± 0.03f
15 EtmH Et H meta 528 541 0.15 1.8 ± 0.1 4.4 ± 0.3f
16 EtmOMe Et OMe meta 527 541 0.05 5.4 ± 0.6 0.60 ± 0.03f
17 TMmH H H meta 503 518 0.11 2.5 ± 0.1 0.62 ± 0.08
18 TMmMe H Me meta 504 517 0.07 6.2 ± 0.4 1.5 ± 0.2
19 TMmOMe H OMe meta 504 512 0.05 33 ± 0.7 1.0 ± 0.1
28 carboxymH COOH H meta 503 516 0.07 4.4 ± 0.2 12 ± 2
29 carboxymMe COOH Me meta 503 517 0.03 9.9 ± 0.4 5.1 ± 0.9
30 carboxymOMe COOH OMe meta 509 522 0.06 24 ± 0.5 7.1 ± 0.8
35 amidemH CONHCH2CO2Me H meta 508 521 0.06 48 ± 2 1.0 ± 0.1
36 amidemMe CONHCH2CO2Me Me meta 509 521 0.03 5.1 ± 0.4 1.0 ± 0.1
22 cyanomH CN H meta 502 519 0.08 3.8e 0.34 ± 0.002
a

Determined in ethanol.

b

Per 100 mV depolarization.

c

Determined in HEK cells.

d

Relative to 19, set to a relative brightness of 1.0.

e

Increased exposure time and light intensity required to make measurement.

f

Pluronic F-127 (0.01%) used during loading.