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. 2020 Jun 9;21:219. doi: 10.1186/s12882-020-01880-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Various key gene expressions concerned with the development of PTGs, Pax1 (a), Pax9 (b), Eya1 (c), Six1 (d), Six4 (e), Gata3 (f), MafB (g), Tbx1 (h), obtained from rats in each group: Control NP rats, Control HP rats, CKD NP rats, and CKD HP rats. All gene expression tended to increase in Control NP rats; however, there was no significant difference. GATA3 and MafB tended to decrease in the CKD NP rats, but did not show any significant difference. In CKD HP rats, the expressions were almost equivalent to that in the Control NP rats. The expressions of other genes tended to decrease in the CKD NP rats and CKD HP rats; however, there was no significant difference compared with the Control NP rats. Gene expression data was measured using real-time RT-PCR and normalized to β-actin. Number of animals: (a) Control NP, 6; Control HP, 6; CKD NP, 6; and CKD HP, 6. Results are presented as the mean ± SD. The mean difference is significant at the 0.0083 level for Bonferroni test