Fig. 4.

Representative immunohistochemical images of specimens stained with Ki67 and counterstained with hematoxylin (× 100) (a) and differences in mean labeling index of Ki67 protein expression (b) among 5DControl NP, 5DControl HP, 5DCKD NP and 5DCKD HP groups. a Nuclear Ki67 expression. Many nuclei in the CKD HP group were positively stained for Ki67. b Ki67 expression significantly increased in the 5DCKD HP group compared with the other groups. Albeit not statistically significant, the number of Ki67-positive cells tended to be higher in the 5DControl HP and 5DCKD NP groups. Number of animals: 5DControl NP, 6;5D Control HP, 6; 5DCKD NP, 6; and 5DCKD HP, 6. Results are presented as mean ± SD. The mean difference is significant at the 0.0083 level for Bonferroni test