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. 2020 Jun 9;21:219. doi: 10.1186/s12882-020-01880-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Gene expression of CaSR (a), VDR (b), and Gcm2 (c) of PTGs in adenine-induced rats for five days, obtained from rats in each group: 5Dcontrol NP rats, 5Dcontrol HP rats, 5DCKD NP rats, and 5DCKD HP rats. The 5DCKD NP rats and 5DCKD HP rats showed a trend of decrease in the expression of the three genes; however, only Gcm2 in 5DCKD HP rats had a significant difference compared with 5DControl groups. Gene expression data were measured using real-time RT-PCR and normalized to β-actin. Number of animals: (a) 5DControl NP, 6; 5DControl HP, 6; 5DCKD NP, 6; and 5DCKD HP, 6. Results are presented as the mean ± SD. The mean difference is significant at the 0.0083 level for Bonferroni test