Fig. 3.

Post-MRI pathology after chronic CPZ. a-f: Coronal sections from naïve and 12 wk. CPZ perfused after final MRI (Fig. 2) and immunolabeled for either myelin (MOG; a-b), astrocytes (GFAP; c-d), or microglia (IBA1; e-f), along with DAPI nuclear stain (blue; c-f). g-j: Quantification of immunolabeling in this post-MRI cohort shows that chronic CPZ results in significant CC atrophy (g) with demyelination (h), astrogliosis (i), and microglial activation (j). CC area was measured using DAPI nuclear staining of cytoarchitecture and MOG immunoreactivity. Bar color reflects condition as naïve (white; no cuprizone), or demyelinated (black; 12 weeks of cuprizone). Data are mean values ± sem. Naïve (n = 6) compared to chronic CPZ (n = 6) using t-tests. Scale bars A, B: shown in A = 200 μm; C, D: shown in C = 100 μm; E, F: shown in E = 100 μm