Figure 1.

CTCL cell lines and primary human SS cells express HDAC6. Primary PBMCs were isolated using standard Ficoll centrifugation and further processed for the magnetic isolation of T-cells. The primary cells were incubated overnight with 100 IU/ml IL-2 and 15 ng/ml IL-15. Established CTCL cell lines were cultured in complete medium without the addition of cytokines. The levels of HDAC6 were assessed by western blot analysis in whole-cell lysates. Tubulin was used as a loading control. The experiments were repeated twice. HD, healthy donor; SS, Sézary syndrome; CTCL, cutaneous T-cell lymphoma; PBMCs, peripheral mononuclear blood cells; HDAC6, histone deacetylase 6.