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. 2020 Apr 21;20(1):326–336. doi: 10.3892/ol.2020.11548

Figure 2.

Figure 2.

CHL1 expression level is correlated with PCNA expression levels in different grades of human gliomas, and downregulation of CHL1 induces glioma cell senescence. (A) Representative immunofluorescence staining of PCNA and CHL1 in CAN and human glioma tissues (graded I–II and II). Scale bars, 50 µm. (B) Correlation between CHL1 and PCNA expression levels (n=23, 18, 85, 27 and 11 for I, I–II, II, III and IV grades, respectively). (C) Immunofluorescence staining of CHL1 and PCNA in U-87 MG cells treated with either a control siRNA or an siRNA targeting CHL1 for 48 h. Downregulation of CHL1 resulted in the reduction of PCNA expression levels. Scale bars, 25 µm. (D) Enhanced staining of senescence-associated β-galactosidase was observed in cells with CHL1 knockdown, suggesting that CHL1 may function by delaying the senescence of glioblastoma cells. CHL1, cell adhesion molecule L1 like; PCNA, proliferation cell nuclear antigen; si, small interfering; con, control.