Figure 4.

Expression levels and localization of CHL1 in U251 human glioma cells and U-87 MG human glioblastoma cells in response to treatment with 2.5 nM NRG1s. U251 and U-87 MG cells were treated with 2.5 nM NRG1α and NRG1β for 48 h. The cells were then fixed and immunostained for CHL1 (green) and p-Neu (red), and then counterstained with DAPI (blue). Representative immunofluorescence staining showing the effects of NRG1α and NRG1β on CHL1 expression levels and localization in (A) U251 and (B) U-87 MG cells., Scale bars 30 and 20 µm, respectively. CHL1, cell adhesion molecule L1 like; NRG1, neuregulin 1; NEU, ErbB2 receptor; p, phosphorylated.