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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Apr 16;40(6):e153–e165. doi: 10.1161/ATVBAHA.120.314360

Figure 1. Notch1+/− murine valves have increased macrophage polarization.

Figure 1.

Flow cytometry was performed on both WT and Notch1+/− aortic valves for CD45 (A), followed by CD11b (B), then F4/80 (C). Macrophages were characterized by Ly6C expression (D), CX3CR1 and CCR2 expression (E, F), and MHCII expression (G, outline = WT; gray fill = Notch1+/−). Non-myeloid hematopoietic cells were grouped by MHCII expression (H), and all cell types were plotted as average percentage of the total CD45+ population (I). Bar plots represent mean ± s.e.m (A-C, F, G). Representative flow plots are of WT animals (A-E, H). N = 8 biological replicates. *P < 0.05 by two-tailed t test.