The extent of aortic atherosclerotic lesion formation, represented by the ratio of lesion area to total aortic area, was quantified by en face analysis of aortae from mice after 3m or 6m on a HFD (Upper). Representative en face graphs are shown (lower) with each vertically matched to the corresponding data set in the upper panel (Student’s t-test, KO vs. Ctrl, * P<0.05, *** p<0.001, n=11–17).