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. 2020 Apr 23;20(1):257–265. doi: 10.3892/ol.2020.11569

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Chemoresistant liver cancer exhibits characteristics of epithelial-mesenchymal transition. (A) E-cadherin mRNA expression levels were significantly reduced in HepG2/ADR cells; however, vimentin and N-cadherin mRNA levels were significantly increased, compared with the parental HepG2 cell line, as demonstrated by reverse transcription-quantitative PCR. (B) Western blotting demonstrated that E-cadherin protein expression was significantly reduced in HepG2/ADR cells; however, vimentin and N-cadherin protein levels were significantly increased compared with the parental HepG2 cell line. Experiments were performed in triplicate. *P<0.05, **P<0.01, ***P<0.001 vs. HepG2. P4HB, prolyl 4-hydroxylase beta polypeptide; ADR, adriamycin.