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Silencing P4HB suppresses the Snail and β-catenin pathways in HepG2/adriamycin cells. (A) Expression levels of total β-catenin, nuclear β-catenin and Snail were significantly reduced in the P4HB siRNA group. Quantitative results of western blots for (B) nuclear β-catenin; (C) total β-catenin; and (D) Snail. Experiments were performed in triplicate. *P<0.05, **P<0.01 vs. NC. P4HB, prolyl 4-hydroxylase beta polypeptide; NC, negative control, siRNA, small interfering RNA.
