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. 2020 May 18;20(1):685–692. doi: 10.3892/ol.2020.11639

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of NPBWR1-2 on SKOV3 cell viability, proliferation, migration, invasion and apoptosis. Cells were transfected with an NPBWR1-2 overexpression or a nonsense pcDNA3.1 control vector. (A) Cell viability was measured using the MTT assay. (B) Cell proliferation was assessed using a colony-formation assay. Cell (C) migration and (D) invasion were assessed using Transwell assays. (E) Apoptotic cells were detected by flow cytometry (the SKOV3/NC group was set at 1 in B, C, D and E). ***P<0.001 and ****P<0.0001. NPBWR1-2, neuropeptides B and W receptor 1–2; NC, negative control; lncRNA, long non-coding RNA.