Table 1.
List of dopamine neuron subtypes and markers with highest variance identified by single-cell gene profiling studies.
| Studies | Developmental stages | DA Subcluster (Markers) |
|---|---|---|
| La Manno et al., 2016 [14] | E11.5–E18.5 | mDA0 (Th+, Cck+, Prkca+) |
| mDA1 (Th+, Dat+, Calb1+) | ||
| mDA2 (Sox6+, Th+, Dat+, Calb1+, Aldh1a1+, Lmo3+, Bnc2+) | ||
| mNbM (Sox6+, Neurod1+, Neurog2+, Ebf2+, Rnd3+, Cnfn2+, Nhlh1+, Igfbpl1+, Pitx3-) | ||
| mNbMDA (Pbx1+, Ebf2+, Cdk14+) | ||
| P28–P56 | mDA-SNC (Sox6+, Calb1−, Aldh1a1+, Aldh1a7+, Anxa1+, Ndnf+) | |
| mDA-VTA1 (Sox6+, Calb1+, Ndnf+) | ||
| mDA-VTA2 (Sox6−, Calb1+, Aldh1a1+, Aldh1a7+, Neurod6+, Grp+, Gpr83+, Otx2+, Lpl+, Anxa1+, Cbln4+) | ||
| mDA-VTA3 (Sox6−, Calb1+, Vip+, Cck+, Cbln1+, Cbln4+) | ||
| mDA-VTA4 (Sox6−, Calb1+, Vgat+, Cbln1+) | ||
| Hook et al., 2018 [16] | P7 | P7.MB.1 (Gpr83+, Otx2+, Cck+, Neurod6+, Lpl+, Tacr3+) |
| P7.MB.2 (Lhx9+, Ldb2+, Dat−, Vglut2+, Wnt7b+) | ||
| P7.MB.3 (Vip+, Calb1+) | ||
| P7.MB.4 (Sox6+, Ndnf+, Lmo3+, Aldh1a1+, Aldh1a7+, Anxa1+, Sncg+) | ||
| E15 | E15.MB.1 (Lhx9+, Ldb2+, Vglut2+) | |
| E15.MB.2 (High Th+, high Dat+, Vmat2+, Pitx3+, Ddc+, En1+) | ||
| Poulin et al., 2014 [13] | P4 | DA1A (Aldh1a1+, Sox6+, Ndnf+) |
| DA1B (Aldh1a1−, Sox6+, Ndnf+, Tacr3+) | ||
| DA2A (Vgat+, Calb1+, Cck+, Vglut2+) | ||
| DA2B (Lpl+, Adcyap1+, Otx2+, Aldh1a1+, Vglut2+, Calb1, Cck+, Grp+, Tacr3+) | ||
| DA2C (Aldh1a1−, Calb1+, Cck+, Vglut2+, Tacr3+) | ||
| DA2D (Vip+, Calb1+, Cck+, Vglut2+, Sox6−, Aldh1a1−) | ||
| Tiklova et al., 2019 [15] | P1-P90 | N-Datlow (Nxph4+, Th−, C1ql1+, Fam19a2+, Car10+) |
| NT-Datlow (Nxph4+, Th+ high, C1ql1+, Fam19a2+, Car10+) | ||
| G- Datlow (Gad2+, Th−, Vgat+, Wnt7b+, Crhbp+) | ||
| GT-Datlow (Gad2+, Th+ high, Crhbp+, Vgat+, Wnt7b+) | ||
| T-Dathigh (Th+ high, Aldh1a1−, Sncg+) | ||
| AT-Dathigh (Aldh1a1+, Th+ high, Otx2+, Grp+, Cck+, Lpl+) | ||
| VT-Dathigh (Vip+, Th+, Dlk1+, Cck+, Gipr+, Pou2f2+) | ||
| Kramer DJ et al., 2018 [17] | P26-P34 | Cluster 1 SNC (Aldh1a7+, Igf1+, Bsn+, Ntsr1+, Kcns3+) |
| Cluster 4 SNC (Aldh1a7+, Igf1+, Bsn+, Ntsr1+, Kcns3+, Anxa1+) | ||
| Cluster 2 VTA (Ubqln2+, Ids+, Slc12a5+) | ||
| Cluster 5 VTA (Necab1+, Cthrc1+, Cbln1+) | ||
| Cluster 6 VTA (Necab1+, Cthrc1+, Cbln1+, Gucy1a3+, Ryr2+, Uri1+) | ||
| Cluster 8 VTA (Neurod6+, Grp+, Gpr83+, Cbln4+, Igfbp4+) | ||
| Saunders et al., 2018 [18] | P60-P70 | SN_3–7 (Vglut2+, Gad2+, Crhbp+, Vgat+, Wnt7b+) |
| SN_4–1 (Lpl+, Vglut2+, Cbln4+, Aldh1a1+, Neurod6+, Otx2+, Calb1+, Tacr3+) | ||
| SN_4–2 (Cbln1+, Vglut2+, Calb1+, Wnt7b+) | ||
| SN_4–3 (Cyp26b1+, Sox6+, Tacr3+) | ||
| SN_4–4 (C1ql3+, Calb1+)* | ||
| SN_4–5 (Vcan+, Aldh1a1+, Sox6+) | ||
| SN_4–6 (Cadm2+)* | ||
| SN_4–7 (Nefl+, Nefm+)* | ||
| SN_4–8 (Aldh1a1+, Anxa1+, Sox6+, Igfbp2+) | ||
| SN_4–9 (Grin2c+, Aldh1a1+, Sox6+) |
Notes:
1) Genes in bold represents those explicitly discussed in the papers, whereas those not in bold represent our own mining of underlying data
2) Asterisk (*) indicates populations of neurons in [18] which we were unable to definitively determine the correspondence to subtypes in other papers, or the localization in the mouse brain.