Table 2.
Key methodological aspects of single-cell dopamine neuron classification studies.
| Studies | Mouseline | Age | Isolation | Technique | # of cells |
|---|---|---|---|---|---|
| Poulin et al., 2014 [13] | Dat-IRES-Cre, Ai9 | P4 | FACS for tdTomato | sc-qPCR | 159 |
| La Manno et al., 2016 [14] | Dat-IRES-Cre, Ai9 | P28-P56 | FACS for tdTomato | scRNA-Seq | 245 |
| Hook et al., 2018 [16] | Th-eGFP | P7 | FACS for eGFP | scRNA-Seq | 80 |
| Saunders et al., 2018 [18] | wildtype | P60-P70 | No sorting | Drop-Seq | 919 |
| Kramer et al., 2018 [17] | Dat-IRES-Cre, Ai9 | P26–34 | FACS for tdTomato | scRNA-Seq | 232 |
| Tiklova et al., 2019 [15] | Pitx3-eGFP | P1-P90 | FACS for eGFP | scRNA-Seq | 1106 |