Fig. 1.

Average coverage of each position in the AAvV-1 viral genome sequences, per method. The viruses were sequenced using three different methods including the specific primers (34), SISPA (57) and 96 samples via NGS sequencing kits (8 IonTorrent, 84 Proton Torrent, 4 Illumina). In all the methods a drop in the coverage near nucleotide 1700 is seen, possibly due to the G-quadruplexes. The Illumina and Proton Torrent have the highest coverage. The lowest coverage was obtained by the specific primers and SISPA methods (sequenced via the Ion Torrent).