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. 2020 Jan 16;5:100027. doi: 10.1016/j.gene.2020.100027

Fig. 1.

Fig. 1

Mechanical strain alters gene expression in Saos-2 cells. Mechanical strain down-regulates SOST and RANKL (TNFSF11) expression, but does not alter OPG (TNFRSF11B) and RUNX2 expression in Saos-2 cells. RT-qPCR analysis of SOST, RANKL, OPG (A) and RUNX2 (B) subjected to strain for 8 h. RT-qPCR analysis of SOST (C) and RANKL (D) of cells pre-treated with 2 μM Actinomycin D or vehicle 1 h before exposure to strain for 8 h. Bars represent the mean ± SEM, n = 10–15. * p < .05, ** p < .01, *** p < .001 versus static controls.