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. 2020 Jan 16;5:100027. doi: 10.1016/j.gene.2020.100027

Fig. 2.

Fig. 2

RUNX2 suppresses expression of SOST. Stable knockdown of RUNX2 in decreases alkaline phosphatase activity and increases SOST expression, but does not alter proliferation and RANKL levels in Saos-2 cells. RT-qPCR analysis of RUNX2 in vector control and shRUNX2 transfected cells (A). Percentage of Ki-67 positive cells (B) and alkaline phosphatase activity (ALP) (C) in vector control or shRUNX2 transfected cells. RT-qPCR analysis of RANKL (D) and SOST (E) in vector control or shRUNX2 transfected cells. Bars represent the mean ± SEM, n = 10–12 with the exception of B (n = 4). ** p < .01, *** p < .001 versus vector controls.