Fig. 3.

Strain-mediated suppression of SOST proceeds independently of RUNX2. RUNX2 influences basal SOST expression but not regulation of SOST by strain, whereas strain-related RANKL down-regulation but not basal expression are influenced by RUNX2. Cells were treated with 1 μM AzadC for 3 days and assessed for SOST (A) and RANKL (B) expression by RT-qPCR analysis (n = 10). Vector control or shRUNX2 cells were treated with vehicle or 0.5 μM PGE2 and harvested 24 h later to assess for RANKL (C) and SOST (D) expression by RT-qPCR analysis (n = 10). Cells were subjected to strain and harvested 8 h later to assess for SOST (E) and RANKL (F) expression by RT-qPCR analysis (n = 15). Bars represent the mean ± SEM. ** p < .01, *** p < .001 versus static controls of the same cell type, ^### p < .001 versus AzadC-treated pWTS1 cells.