Figure 5.

Requirement of caspase activation in dasatinib-induced pyroptosis. (A) Suppression of GSDME cleavage by pretreatment with caspase-3 inhibitor zDEVD when the SH-SY5Y cells were treated with 40 µm dasatinib. (B) Caspase-3 activity in A549 cells could not be inhibited by caspase-3 specific inhibitor zDEVD. (C) Inhibition of GSDME cleavage by pan-caspase inhibitor zVAD when the A549 cells were treated with 30 µm dasatinib. One representative result from three independent experiments is shown. *P<0.05, **P<0.01 represents the drug treated groups vs. control group. GSDME, gasdermin E; GSDME-N, N-terminal fragment of GSDME; zDEVD, caspase-3 inhibitor Z-DEVD-FMK; zVAD, pan-caspase inhibitor Z-VAD (OMe)-FMK; CASP3-C, cleaved caspase-3.