Table 4.
Summary of the serum biochemistry and histological lesions that resulted from orogastric doses of 1-cyano-2-hydroxy-3-butene (CHB) and 1-cyano-2-hydroxy-3,4-epithiobutane (CHEB) in rats in this pilot trial.
| Compound | Dose (mmol/kg); no. of daily doses; time to death or euthanasia | Pancreas enzymes | Pancreas lesions | Liver enzymes | Liver lesions | Serum creatinine | Kidney lesions | Stomach lesions | Small intestine lesions |
|---|---|---|---|---|---|---|---|---|---|
| CHB | 1; 1; 24 h–14 days | – | ++++ | – | – | – | – | – | – |
| “ | 1; 2–5; 48 h–14 days | – | ++++ | – | + | – | – | – | – |
| “ | 2; 1; 8–10 h | ++++ | ++ | ++ | + | – | – | – | – |
| “ | 3; 1; 9 h | +++ | ++ | ++++ | ++ | – | – | – | + |
| CHEB | 1; 1; 24 h–14 days | – | – | – | – | ++ | +++ | + | – |
| “ | 1; 2–3; 48–68 h | – | – | ++ | ++ | ++ | ++++ | ++ | – |
| “ | 2; 1; 5 h | – | + | +++ | ++ | +++ | + | – | – |
| “ | 3; 1; 4 h | – | + | ++ | + | +++ | + | + | + |
| CHB + CHEB | 1; 1–5; 24 h–14 days | – | – | – | + | + | ++ | ++ | – |
|
CHB + CHEB + minor nitriles |
1; 1–5; 24 h–14 days | – | – | – | + | – | ++ | ++ | – |
“-” = no lesions or serum biochemical changes.