Table 5.

Free cyanide concentrations in livers from rats dosed with varying concentrations of nitriles. Samples were analysed using gas chromatography, with a method limit of detection (MDL) of 0.01 μg/g.

Rat #	Treatment	Final humane endpoint score	Time interval between dose and death/euthanasia (h)	Cyanide (μg/g)
1	CHB 1 mmol/kg	0	24	<MDL
2	CHB 1 mmol/kg	0	48	<MDL
7	CHB 2 mmol/kg	4	8a	0.01
8	CHB 2 mmol/kg	4	10	0.01
9	CHB 3 mmol/kg	4	9	0.01
10	CHB 3 mmol/kg	4	9a	0.02
16	CHEB 1 mmol/kg	0	24	<MDL
17	CHEB 1 mmol/kg	0	48	<MDL
22	CHEB 2 mmol/kg	2	11	<MDL
23	CHEB 2 mmol/kg	8	5	<MDL
24	CHEB 3 mmol/kg	2	24	<MDL
25	CHEB 3 mmol/kg	8	4a	<MDL
37	3-BN 1 mmol/kg	6	18.5	0.5
38	4-PN 1 mmol/kg	0	24	0.01
39	4-PN 1 mmol/kg	2	48	0.01
40	5-HN 1 mmol/kg	0	24	<MDL
41	5-HN 1 mmol/kg	0	48	<MDL
48	Control - no treatment	0	24	<MDL
49	Control - no treatment	0	48	<MDL
50	Control - emulsion	0	24	<MDL
51	Control - emulsion	0	48	<MDL

^aRats were found dead, or in extremis in the case of Rat 25, at the set monitoring time. The reported final humane endpoint score is the score given to the rat at the last monitoring check, 3 h prior.