Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 15;20(1):235–247. doi: 10.3892/ol.2020.11534

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — lncRNA-CD160 expression is positively associated with CD160 expression in CD8⁺ T cells. (A) lncRNA gene microarray assay was conducted with CD160⁺ CD8⁺ T cells and CD160⁻ CD8⁺ T cells, which were isolated from patients with chronic HBV, with unsupervised clustering analysis. Green indicates decreased expression and red indicates increased expression. (B) lncRNA gene microarray assay with supervised clustering analysis was performed with CD160⁺CD8⁺ T cells and CD160⁻ CD8⁺ T cells. (C) Reverse transcription-qPCR assay was performed to detect the lncRNA-CD160 expression level in CD160^+/− CD8⁺ T cells. (D) Chromosome analysis indicated that both CD160 and lncRNA-CD160 were located at Chr1q42.3, and lncRNA-CD160 was partly located at the region of CD160, which was between the B and C region; therefore, lncRNA-CD160 could also be termed lncRNA-CD160. Chromatin immunoprecipitation-qPCR was performed to investigate the relationship between (E) lncRNA-CD160 and H3K9Me1, (F) the relationship between lncRNA-CD160 and HDAC11 also was detected. HDAC11 and H3K9Me1 trimethylation levels were promoted in the lncRNA-CD160 loci. *P<0.05, **P<0.01, ***P<0.005. qPCR, quantitative PCR; lncRNA, long non-coding RNA; HBV, hepatitis B virus; HDAC11, histone-modification enzyme gene histone deacetylases 11.