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. 2020 Jun 3;11:746. doi: 10.3389/fpls.2020.00746

FIGURE 3.

FIGURE 3

H+-ATPase activity, net photosynthetic rate, and RuBisCO activity in Al stressed alfalfa seedlings. (A) H+-ATPase activity in shoots of alfalfa seedlings with apical buds grown in 1.5 mM Ca(NO3)2 medium (pH 4.5) containing 0 μM AlCl3 (pH4.5), 100 μM AlCl3 (pH4.5+Al), 100 μM AlCl3 and 25 μM MgCl2 (pH4.5+Al+25 μM Mg), and 100 μM AlCl3 and 50 μM MgCl2 at the third day after the initiation of treatments. (B) Net photosynthetic rate and (C) RuBisCO activities in leaves of alfalfa seedlings with apical buds grown in 1.5 mM Ca(NO3)2 medium (pH 4.5) containing 0 μM AlCl3 (pH4.5), 100 μM AlCl3 (pH4.5+Al), 100 μM AlCl3 and 50 μM MgCl2 (pH4.5+Al+Mg), 100 μM AlCl3 and 6 mg L–1 IAA (foliar spray) (pH4.5+Al+IAA), or 100 μM AlCl3 and 50 μM MgCl2 and 6 mg L–1 IAA (foliar spray) (pH4.5+Al+Mg+IAA) at the third and sixth days after the initiation of treatments. Data are means ± SE of three replicates from three independent experiments. Bars with different letters indicate significant difference at P < 0.05 (least significant difference test).