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. 2020 Jun 3;11:746. doi: 10.3389/fpls.2020.00746

FIGURE 8.

FIGURE 8

Parameters derived from the dark-interval relaxation kinetic of ECS (DIRKECS) in Al stressed seedlings with or without apical buds. Five treatments in the seedlings with apical buds are as Figure 1, and seedlings without apical buds are grown in 1.5 mM Ca(NO3)2 medium (pH 4.5) and treated with or without spraying IAA (pH4.5-IAA, pH4.5+IAA), 100 μM AlCl3 with or without spraying IAA (pH4.5+Al-IAA, pH4.5+Al+IAA), and 100 μM AlCl3 and 50 μM MgCl2 with or without spraying IAA (pH4.5+Al+Mg-IAA, pH4.5+Al+Mg+IAA). Total proton motive force (pmf) (A), the proton gradient (ΔpH) (B), and the thylakoid proton conductivity (gH+) (C) were estimated from seedlings with apical buds, and pmf (D), ΔpH (E), and gH+ (F) were estimated from seedlings without apical buds at the third day after the initiation of treatments. Bars with different letters indicate significant difference at P < 0.05 (least significant difference test).