Digestion buffer	Stock solution concentration	Amount (ml)	Final concentration (/100 ml solution)
Triton X‐100	5% (w/v)	10	0.50 g
Ethylenediaminetetraacetic acid (EDTA; Thermo Fisher, cat. no. 15575020)	0.5 M, pH 8.0	0.2	0.2 ml (1 mM)
Tris(hydroxymethyl)aminomethane (Tris; Thermo Fisher, cat. no. AM9855G)	1 M, pH 8.0	5	5 ml (50 mM)
Sodium chloride	5 M	20	4.67 g (1 M)
Water		64.8
Total		100