Microtubule fixation solution	Amount (ml)	Final concentration
16% (w/v) paraformaldehyde (Electron Microscopy Sciences, cat. no. 15710)	1.5	3% (w/v)
8% (w/v) glutaraldehyde (Electron Microscopy Sciences, cat. no. 16019)	0.1	0.1% (w/v)
10× PBS (Corning, cat. no. 21040CV))	0.8	1×
Water	5.6
Total	8