Monomer solution (“Stock X”)	Stock solution concentration (g/100 ml solution)	Amount (ml)	Final concentration after addition of APS and TEMED stock solutions (g/100 ml solution)
Sodium acrylate (see vendors below)	38 (33% (w/w) in water)	2.25	8.6
Acrylamide (Sigma Aldrich, cat. no. A9099‐25G)	50	0.5	2.5
N,N′‐Methylenebisacrylamide (Sigma Aldrich, cat. no. 146072‐100G)	2	0.75	0.15
Sodium chloride (Thermo Fisher, cat. no. AM9759)	29.2 (5 M)	4	11.7 (2 M)
PBS	10×	1	1×
Water		1.3
Total		9.8