Fig. 2.

Effect of BaTX-II, isolated from B. atrox snake venom on human neutrophils hydrogen peroxide production. 2 × 10⁵ neutrophils were incubated for 1:30 h with BaTX-II at concentrations (1.5, 3, 6, 12.5 and 25 μg/mL) or RPMI (negative control) or PMA (500 ng/mL; positive control) at 37 °C and 5% of CO₂. After incubation, the hydrogen peroxide production was determined in the presence or absence of wortmannin, a PI3K inhibitor, after reading the optical density at 620 nm. The results were expressed as μM H₂O₂. Data represent the mean ± SEM of 5 independent donors. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to negative control. ###P < 0.001, ####P < 0.0001 compared to respective experimental without inhibitor (ANOVA).