Figure 4.

AICAR downregulates the physical interaction between eEF2K and MEK1/2. Immunofluorescence confocal microscopy of eEF2K and MEK1/2 in MKN45 cells treated under ND with and without AICAR (400 µM) for 3 h. The cells were incubated with primary antibodies against eEF2K or MEK1/2 and then with fluorescent secondary antibodies conjugated with AlexaFluor 555 (red) and 488 (green) for eEF2K and MEK1/2, respectively. Scale bar, 25 µm. AICAR, acadesine; eEF2K, eukaryotic elongation factor 2 kinase; MEK1/2, dual-specificity mitogen-activated protein kinase kinase 1/2; ND, nutrition deprivation.