Table 3.
Intra- and inter-assay reproducibility of multiplex real-time PCR.
Assay | DNA (copies/μL) | Intra-assay |
Inter-assay |
||||
---|---|---|---|---|---|---|---|
Mean Cq | SD | CV (%) | Mean Cq | SD | CV (%) | ||
CPV | 107 | 16.66 | 0.01 | 0.07 | 16.83 | 0.15 | 0.88 |
106 | 19.94 | 0.02 | 0.09 | 20.11 | 0.15 | 0.73 | |
104 | 27.06 | 0.18 | 0.68 | 27.21 | 0.16 | 0.59 | |
103 | 30.52 | 0.19 | 0.62 | 30.52 | 0.21 | 0.67 | |
CCoV | 107 | 15.81 | 0.01 | 0.06 | 15.89 | 0.07 | 0.45 |
106 | 19.23 | 0.02 | 0.11 | 19.41 | 0.16 | 0.82 | |
104 | 26.09 | 0.25 | 0.98 | 26.26 | 0.15 | 0.58 | |
103 | 30.66 | 0.18 | 0.60 | 30.71 | 0.23 | 0.74 | |
CaKoV | 107 | 13.46 | 0.02 | 0.13 | 13.58 | 0.14 | 1.00 |
106 | 17.28 | 0.01 | 0.03 | 17.36 | 0.07 | 0.42 | |
104 | 24.44 | 0.09 | 0.36 | 24.55 | 0.29 | 1.20 | |
103 | 28.14 | 0.28 | 0.98 | 27.95 | 0.62 | 2.22 | |
CAstV | 107 | 14.02 | 0.04 | 0.25 | 14.20 | 0.18 | 1.29 |
106 | 17.61 | 0.03 | 0.14 | 17.70 | 0.08 | 0.44 | |
104 | 24.00 | 0.19 | 0.77 | 24.20 | 0.18 | 0.74 | |
103 | 28.05 | 0.20 | 0.70 | 28.24 | 0.17 | 0.61 |