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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: J Am Chem Soc. 2020 Mar 5;142(11):5241–5251. doi: 10.1021/jacs.9b13406

Figure 2. eEndoV binding favors ssRNA over dsRNA substrates.

Figure 2.

a) Schematic of dsRNA target annealing and b) duplex verification by 10% native PAGE. c) MST analysis of eEndoV binding affinity towards dsRNA A and d) dsRNA I targets using MST. Values represent mean with standard deviation. (n = 3) e) Representative PAGE analysis of initial (I), flowthrough (FT) and eluate (E) EndoVIPER fractions when tested with various dsRNA targets. f) Densitometric analysis of EndoVIPER efficiency for dsRNA targets. Values represent mean with standard deviation (n = 2). Unpaired t-test was performed for all samples against ssRNA I pulldowns (*** denotes p = 0.0003 and **** denotes p < 0.0001).