Fig. 3. Becn1^F121A rescues cardiac and renal phenotypes in kl/kl mice.

(A) Cardiac phenotypes in 4 genotypes (kl/kl, WT, BK/BK;kl/kl, and BK/BK mice) at the age of 10 weeks. Representative macrographs of heart sections stained with Trichrome. Bottom panel is summary data of heart weight over body weight. Scale bar = 2 mm. Number of mice of each genotype is shown in parenthesis at the bottom. (B) Representative microscopic images of WGA stained left ventricle sections of 5 mice from each genotype. Bottom panel is summary data. with scatter plots of individual data points. Scale bar = 50 μm. (C) Representative immunoblots of total left ventricular lysates for α-actinin and α-SMA αKlotho protein in the heart of 5 mice from each genotype. Right panel is a summary of all immunoblots. (D) Vascular calcification in 4 genotypes at 10 weeks old. Representative microscopic images of Von Kossa stain in the aortic roots of 4 mice from each genotype. Scale bar = 200 μm. (E) Trichrome stain in the kidney sections of 4 genotypes at the age of 10 weeks. Representative microscopic images of Trichrome stain in the kidney sections of 6 mice from each genotype at 10 weeks. Scale bar = 25 μm. (F) Fibrotic marker, α-SMA protein expression in the kidneys. Upper panel shows representative immunoblots of total kidney lysates for α-SMA protein expression. Bottom panel is summary of data from 5 mice for each genotype. (G) Representative microscopic images of ectopic calcification in Von Kossa stained kidney sections from 4 mice for each genotype. Scale bar = 500 μm. Data shown in A - C, and F are means ± S.D. with scatter plots of individual data points. *P<0.05, **P<0.01 between two groups by one-way ANOVA followed by Student-Newman-Keuls post hoc test for A - C, and F.