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. Author manuscript; available in PMC: 2020 Jun 10.
Published in final edited form as: FASEB J. 2020 Jan 5;34(2):3129–3150. doi: 10.1096/fj.201902127R

Fig. 4. Early effect of autophagy on Pi homeostasis and mouse growth is independent of αKlotho expression in kl/kl mice.

Fig. 4.

(A) Experimental design. kl/kl mice at 4 weeks were intraperitoneally given rapamycin (Rapa), chloroquine (CQ), recombinant αKlotho protein (αKlotho) or vehicle (normal saline) through osmotic minipumps. Four weeks after intraperitoneal administration, mice were sacrificed for B - E. Each group had 6 mice with equal male vs female number. (B) Body weight is presented as means from 6 mice per group. *P<0.05; **P<0.01 vs vehicle group between two groups by one-way ANOVA followed by Student-Newman-Keuls post hoc test at indicated time point. (C) Plasma Pi of mice. Data are presented as means ± S.D. with scatter plots of individual data points for 6 mice per group. (D) Representative immunoblots of total kidney lysates for αKlotho, LC3 and p62 protein expression in the kidneys of 5 mice from each group. Right panel is summary of data from all blots. Data are presented as means ± S.D. with scatter plots of individual data points for 5 mice per group. *P<0.05, **P<0.01 between two groups by one-way ANOVA followed by Student-Newman-Keuls post hoc test for C and D. (E) Representative microscopic images of Trichrome stained kidney sections from 6 mice randomly selected from each group. Scale bar = 200 μm.