Table 1.
Effect of high Pi diet on plasma αKlotho, cFGF23, and 1,25-(OH)2D
| αKlotho (pmol/l) | cFGF23 (RU/ml) | 1,25-(OH)2D (pg/ml) | ||
|---|---|---|---|---|
| Normal Pi diet | ||||
| WT | 45.6±3.5 (14) |
105.7±28.2 (12) |
104.5±13.4 (14) |
|
| BK/BK | 48.7±4.1 (14) |
139.5±26.4 (12) |
96.5±4.6 (12) |
|
| BK/BK;kl/kl | 41.2±3.5 (12) |
144.5±32.3 (14) |
113.4±12.5 (14) |
|
| P value | >0.05 | >0.05 | >0.05 | |
| High Pi diet | ||||
| WT | 31.1±2.3 (14) |
347.4±35.0 (14) |
277.2±31.6 (16) |
|
| BK/BK | 42.2±3.3**
(16) |
353.6±45.6 (12) |
124.2±21.4**
(14) |
|
| BK/BK;kl/kl | 30.2±3.0##
(10) |
431.7±84.3*#
(10) |
347.4±42.1**##
(10) |
|
| P value | <0.01 | <0.05 | <0.01 |
Three mouse lines (WT, BK/BK, and BK/BK;kl/kl) were fed with normal Pi (0.7%) or high Pi (2.0%) diet for 6 weeks starting at 6 weeks old. The ratio of female and male mice were 50:50. After 6 weeks of dietary Pi challenge, mice were terminated and plasma was harvested for the measurement of soluble αKlotho, C-terminal FGF23, and 1,25-(OH)2D. Data are presented as means ± S.D., the number in parentheses is sample size. The statistical analysis was conducted with one-way ANOVA followed by post hoc tests for any two groups.
P<0.05
P<0.01 vs WT mice
P<0.05
P<0.01 vs BK/BK mice by one-way ANOVA followed by Student-Newman-Keuls post hoc test. cFGF23: c-terminal fragments of fibroblast growth factor-23; kl/kl: homozygous αKlotho hypomorphs; Pi: inorganic phosphate; 1,25-(OH)2D: 1,25-dihydroxyl-vitamin D; WT: wild type.