Figure 6. Kidney-specific Xbp1s knockdown associates with decreased expression of the pro-apoptotic transcription factor CHOP.
qPCR and immunoblot of the pro-apoptotic marker Chop/CHOP in the kidney of tissue-specific Xbp1s knockdown (A-B) and KIXs (C-D) mice at 24 hours after an intraperitoneal (IP) injection of LPS (16 mg/kg) or vehicle. Fold change is relative to vehicle-treated control mice. KIXs and control mice received 0.1 mg/ml Dox- containing water for 16 hours prior LPS injection. Bars and error bars are mean and SEM. 2-way ANOVA, Sidak’s multiple comparisons test. * indicates P<0.05, ** indicates P<0.01, *** indicates P<0.001. LPS: lipopolysaccharide.