KIXs and control mice received 0.1 mg/ml Dox-containing water for 16 hours, then an intraperitoneal (IP) injection of LPS 16 mg/kg (control: n=5; KIXs: n=5) or vehicle (control: n=4; KIXs: n=4), and sacrificed 24 hours later. (A) Serum Cr levels from control or KIXs mice injected with vehicle or LPS. (B) NGAL protein expression normalized by β-actin in the kidney of KIXs mice compared to controls. (C) qPCR on kidney samples from mice injected with vehicle or LPS. Fold change is relative to vehicle-treated control mice. Il6 mRNA expression was undetectable in the kidney of mice treated with vehicle. Bars and error bars are mean and SEM. 2-way ANOVA, Sidak’s multiple comparisons test. * indicates P<0.05, ** indicates P<0.01, *** indicates P<0.001. Cr: creatinine; LPS: lipopolysaccharide.