Figure 4.

LIMD1‐AS1 impeded proliferation and prompted apoptosis through LIMD1 in non‐small cell lung cancer. A549 cells were transfected with sh‐NC, sh‐LIMD1‐AS1#1, sh‐LIMD1‐AS1#1 + pcDNA3.1, or sh‐LIMD1#1 + pcDNA3.1/LIMD1, respectively. A, qRT‐PCR and western blot data of LIMD1 level in A549 cells of each group. B‐C, CCK‐8 and EdU assays were used to monitor the proliferation of A549 cells of each group. D, Caspase‐3 activity was evaluated in A549 cells of each group. E, Apoptotic A549 cells in each group was analyzed by flow cytometry. *P < .05, **P < .01