TABLE 1.

Human gene primers used in quantitative real‐time PCR

Name	Gene no.		Gene length (bps)	Primer sequence 5′‐3′	Amplicon (bps)	Annealing temperature
MMP2	NM_004530.6	1982		F: CAACTACGATGATGACCGCAA	140	62
				R: GTGTAAATGGGTGCCATCAGG
MMP9	NM_004994.3	2124		F: TTGACAGCGACAAGAAGTGG	179	62
				R: GCCATTCACGTCGTCCTTAT
β‐actin	NM_001101.5	1127		F: TCACCCACA CTGTGCCTATCTACGA	295	62
				R: CAGCGGAACCGCTCATTGCCAATGG

Abbreviations: MMP2, matrix metallopeptidase 2; MMP9, matrix metallopeptidase 9; PCR, polymerase chain reaction.