Fig 1. The catalytic activity of Mus81 and Mms4 are required for cell viability and growth in the absence of telomerase.
Serial dilution assays monitoring cell density after 24 hours from an initial inoculate of 5x105 cells. For A-C, average cell density is plotted with one standard of error for (A) wild type (WT) (n = 20), est2Δ (n = 40), and est2Δ mus81Δ (n = 27), as well as (B) est2Δ mms4Δ (n = 8) and est2Δ mus81-D414,415A (n = 23). The Mus81 catalytic mutant, mus81-D414,415A, is represented by mus81-dd. (C) Additional analysis was completed on endonuclease mutants est2Δ rad1Δ (n = 7), est2Δ yen1Δ (n = 15), and est2Δ slx1Δ (n = 24). (D) The lowest average cell density was plotted for all strains analyzed in A-C. Statistics compare mean values to est2Δ single mutant. Haploid strains est2Δ mus81Δ, est2Δ mms4Δ were generated by sporulation of WDHY2961, as described in Materials and Methods. Similarly, est2Δ mus81-dd was derived from WDHY3007. Haploid strains est2Δ slx1Δ, est2Δ yen1Δ, and est2Δ rad1Δ were derived from WDHY3027, WDHY3036, and WDHY3145 diploids respectively. Additional WT and est2Δ haploids were derived from all five diploids. Student T-test is * P < 0.05, ** P < 0.01.