Fig 2. Loss of Mus81 in the absence of telomerase results in reduced cell viability.

(A) Average relative viability with one standard error is presented for wild type (WT) (n = 20), est2Δ (n = 40), and est2Δ mus81Δ (n = 26). (B) Relative cell viability was calculated at the generation with the lowest average cell density (senescence crisis). The Mus81 catalytic mutant, mus81-D414,415A, is represented by mus81-dd. Statistics compare mean values to est2Δ single mutant. Haploid strains est2Δ mus81Δ, est2Δ mms4Δ were generated by sporulation of WDHY2961, as described in Materials and Methods. Using a similar method, est2Δ mus81-dd was derived from WDHY3007. Haploid strains est2Δ slx1Δ, est2Δ yen1Δ, and est2Δ rad1Δ were derived from WDHY3027, WDHY3036, and WDHY3145 diploids respectively. Additional WT and est2Δ haploids were derived from all five diploids. Student T-test is * P < 0.05, ** P < 0.01, *** P < 0.001.